Five experimenters entered the observation stage.

The five groups of rats were numbered as Han Meng group, Cui Hualin group, Tang Yaxing group, Du Yuan group, and Wu Junqiang group, and half of them were male and female.

The miracle did not happen for the time being, but the tragedy happened soon.

After the anesthesia effect of the Duyuan group rats ended, they died one after another within one day.

The experimental records are:

The No. 1 mouse in Duyuan Group died at 12:42 and suffered intracranial hemorrhage.

The No. 2 mouse in Duyuan Group died at 12:43 and suffered intracranial hemorrhage.

...

The No. 10 mouse in Duyuan Group died at 12:51 and suffered intracranial hemorrhage.

To be precise, 10 experimental mice in Du Yuan's group died one after another within ten minutes, and one died on average in one minute.

The causes of death are the same, both of which are deaths due to intracranial hemorrhage.

"Oh, I failed." Du Yuan was very upset. His experimental plan was proven to have failed completely. His ten friends died suddenly before they could do an IQ test and died tragically in the laboratory.

Du Yuan could only be upset for a minute. He had to race against time to dissect the 10 dead experimental mice. The main work was to analyze the brain tissue of the experimental mice, summarize the experience and lessons, and accumulate valuable data for the next experimental design.

There are indeed risks in performing gene editing experiments on living organisms.

Du Yuan only modified a gene of the experimental mice, which caused his experimental mice to be wiped out in a very short period of time.

"Brother, don't be discouraged. Failure is the mother of success." Han Meng comforted Du Yuan, and he said: "You can sort out the analysis report of your experimental data as soon as possible and give your conclusions. Is there a problem with the homologous recombination editing method, or is there a problem with your path selection based on this method? We can only adopt the exclusion method, trial and error one by one until we find the relatively correct method and specific path."

While Du Yuan summarized his experiences and lessons, the other four experimenters continued to observe the rats in their group.

Soon after, Shen Qi joined the ranks of observers. He noticed that the rats in Cui Hualin's group became very manic. In this group of rats, they would bite the same type if they could not bite people, and they would bite the metal mesh in the cage if they could not bite the same type.

"Squeak!"

The rats in Cui Hualin's group grinned and looked ferocious. Now they don't like fresh fruits and vegetables, they just want to bite live things.

"Oh, I failed." Cui Hualin sighed. His friend was extremely crazy after gene editing and could not do IQ tests at all. The craziest rat actually bit off its front foot.

The smell of blood made the rat even more crazy. It hit the metal mesh violently, trying to break through the cage to bite the living creature outside the cage.

Shen Qi patted Cui Hualin on the shoulder: "Extract living cells, preserve brain tissue, and then execute them."

"Director Shen, why don't we observe for a while?" Cui Hualin was unwilling to fail like this, and he still had a glimmer of hope.

Shen Qi shook his head: "No need to continue observing, kill all the experimental mice in your group. I want to see their original data. If I expected, the modification of their shank3 gene affects their neurons. This effect is irreversible, and they may become even more crazy."

"Okay..." Cui Hualin executed his little friend with tears in his eyes.

Before the execution, Cui Hualin first used atomization and anesthesia to faint his friend, extracted living cells, then dislocated and executed, dissected and processed, and preserved various organs and brain tissue.

There is no good news, but bad news comes one after another.

The rats in Tang Yaxing group did not show crazy aggressiveness, but they became extremely autistic. They curled up in the corner of the cage and remained motionless, like a still mouse with hair.

"Your group of autistic experimental mice cannot conduct subsequent experiments, extract living cells, save brain tissue, and then execute them. I want to see their original data." Shen Qi issued the order.

"What a pity, what a pity." Tang Yaxing shook his head and sighed, and then executed his friend.

Currently, one group of rats died due to their own reasons, and the other two groups of rats with abnormal performance were executed by the experimenter.

The progress of human science is inseparable from the contributions made by these experimental animals.

Scientific research continues, and the rats continue to contribute to the progress of human science in their short lives.

After observation and various physical examinations, the experimental mice in Han Meng's group and Wu Junqiang's group were temporarily normal in terms of physical indicators and animal behavior, and can undergo t-maze tests.

I don’t know if it’s good news or bad news, but the T-maze test scores of Wu Junqiang’s experimental mice in the group are consistent with those before.

This group of rats encountered a t-shaped intersection in the T-maze, and their choice was always to go to the right.

Most rodents have a one-way preference characteristic, and the wistar rats selected by Shen Qi's team prefer to the right.

The route of the T-maze designed by Wu Junqiang is eighteen right and one left.

Wistar rats can walk out of the maze by 18 consecutive times and then to the left once, thereby obtaining food placed at the exit of the maze.

The spatial learning ability of the rats in Wu Junqiang's group has not become stronger. They are quite iron-headed, and they keep going to the right and never to the left.

In other words, the IQ of Wu Junqiang's experimental mice who had been edited by shank3 genes remained unchanged and was still at an ordinary level.

"The experimental mice in Wu Junqiang's group are worthy of continuing research." Shen Qi made the above instructions.

"Okay, understand!" Wu Junqiang rubbed his hands excitedly. His friends would not be executed for the time being. His experimental plan was not proven to be the most appropriate plan, but it was not completely denied.

The five experimenters personally selected their rats, took care of them, and fed them personally, and they had more or less affection for their friends.

Life for a moment is also life. If you can give your friends a way out, even if you live a few more days, the experimenters would not be willing to execute them with their own hands immediately.

Thirty rats were executed and ten rats were retained. At present, the best in the Han Meng group rats were in the best condition.

Han Meng designed a brand new T-maze route, and the results achieved by his first three experimental mice in the maze were better than those before they received shank3 gene editing.

"Han Meng's group of experimental mice is very promising." Shen Qi finally waited for a seemingly good news.

"Brother Meng's group of rats have become stronger in their learning ability!" Other experimenters watched.

The No. 4 rat from Han Meng's group entered the T-maze, which was a female rat.

Scientists have verified that female rats in the category of wistar rats are smarter than male rats.

Why female mice are smarter? The essential reason needs further confirmation.

But even the smart wistar female mice have obvious right-facing preference.

Before doing gene editing, Han Meng group No. 4 female rat could only walk out of the full right-facing T-maze.

In this new T-maze, Han Meng sets two left-facing correct T-junctions.

After the first three female rats failed several times, they remembered the correct route and the wrong route, and they walked out of the maze and ate the food.

The No. 4 female rat performed even better. It failed twice before walking out of the maze.

The right-oriented preference of female rat No. 4 is still the main feature, but at the same time, its learning ability has become significantly stronger.

When it realizes that it will not get food when it goes to the right, it will make a change, choosing to go left at the same intersection.

Cui Hualin was amazed: "Rat No. 4 is quite smart! If you fail once at an intersection, it can remember that the left direction is correct and choose left direction in the next attempt! Meng Ge, it seems that your gene editing method is very reliable!"
Chapter completed!