Han Meng began to edit the rat's shank3 gene. At this time, the nucleotide strands of the rat have not been methylated yet, and the complementary strands contain methylation groups.

These methylations are non-mutant and have the same pairing properties as normal rat nucleotides.

Since there is a time difference between DNA replication and methylation of the sub-strand, this provides Han Meng with the opportunity to scan mismatched bases.

In the in vitro cell-free system of organisms, DNA is used as a template to mimic the genes that control transcription in the organism. Such operations are very expensive.

Expensive precision instruments are fixed assets of the laboratory. Over time, these fixed assets are depreciating. In addition to the consumption of various raw materials, consumables, and water and electricity, five experimenters conducted an experiment for a day, and it would cost tens of thousands of yuan regardless of whether they produced results.

As the experiment progresses further, the money will burn more and more.

The Central Biological Laboratory's scientific research budget this year is RMB 300 million, and it just ended in the first quarter and has spent more than 100 million.

It may not necessarily result after burning money. The four major pits of biochemical titanium rings may be what they mean.

The scientific research funds provided by the state and the sponsorships that Shen Qi received outside, the five experimenters were foolishly unable to tell the difference. What they knew was that the level of money burning in this laboratory was international.

Huahan Gene's laboratory is even more expensive, and its level of money is n times that of Shen Qi's laboratory.

But the labs in universities cannot be compared with those outside companies, and this cannot be compared.

When doing biological research, without sufficient funds, researchers will not be able to realize their academic ideals and life dreams.

One of the most important tasks of a lab owner is to make money.

The five experimenters could understand that Director Shen was not easy, Director Shen was under pressure, and Director Shen was in the process of making more money to maintain the operation of the biological laboratory, Director Shen ran around and worked tirelessly.

The central physics laboratory has now achieved a break-even, and the patent value they created has recovered the investment cost and even had a small surplus.

Chemical laboratories mainly study chemical drugs. Their cost-recovery cycle is long and they are now in a struggle stage where input exceeds output.

What the five biological dogs in the center can do now is to come up with some valuable research results that are widely recognized by the biological community as soon as possible and can create wealth.

Han Meng had the strongest desire to produce results. He operated the instrument calmly and meticulously and observed the molecular level experiment progress.

"Muts recognize mismatched bases. Muth recognizes two different single strands by binding to the unmethylated 5’-gatc-3’ sequence... The effect of mutl is not obvious. It should be to coordinate the activity of the other two proteins and make the deviation caused by muth binding near the mismatch site recognized by muts?"

Han Meng's process of working in the laboratory is also a process of continuous thinking. There are many difficulties and puzzles in the research on rat IQ genes.

Solving difficulties and solving puzzles requires the experimenters to summarize experience from a large amount of experimental data and keenly discover the key key.

If one road cannot be completed, then try another one.

The mismatch editing method was also adopted, but Han Meng's editing path was adjusted. He controlled Muth to cut off the 5' phosphate bond of the G base connected to the methylation sequence, and the DNA helicase then unbuttoned the double strand, and then degraded the exposed single strand by exonuclease, and continued to the downstream of the mismatch site.

The other four brothers used different editing methods to try different experimental routes, supplemented by computer analysis, and each came up with a preliminary plan that they thought was feasible.

Whether it is really effective, please invite the rat to appear.

Rats are big white rats. Shen Qi's team raises the most widely used wistar rats in animal experiments.

The feeding room is next to the biological laboratory, with constant temperature and humidity here, and there is food and drink. The living conditions and quality of life of the rats are maintained at a high level.

Wistar female rats are very fierce during breastfeeding and often take the initiative to bite the hands of the staff who are put into the squirrel cage when feeding.

Under normal circumstances, these rats are more docile and can be raised in large quantities in a cage.

Han Meng and five other experimenters entered the breeding room. The rats in the clever group were ready to move, rubbing their front paws off the ground, as if they were trying to look flattering.

The rats in the ordinary group rolled their small eyes and their noses and sniffed something. They felt the arrival of non-similar creatures.

The rats in the fool group squeezed into a group of silly and remained indifferent.

Everything has spirituality, and people who study animals must first understand animals.

Cui Hualin observed them in front of the cage of ordinary rats, and he suggested: "Before gene editing, please ask them to have a good meal."

"This is what it should be." Han Meng nodded, and then arranged for the rats to eat the good ones.

The feed for experimental white rats has national standards, and these rats usually eat white rat feed that meets the national standards.

Although white rat feed is rich in nutrients, it has fewer flavor choices, and only one or two flavors are eaten after eating.

These rats love to eat fresh cabbage, cucumber, apple, sunflower seeds, black currants, cherries and other fruits and vegetables.

The five experimenters each selected a cage of ordinary rats and invited their friends to eat fresh fruits and vegetables.

The ordinary group of rats who ate the delicious food were very happy. They jumped back and forth and made hearty laughs.

Although it sounds like a squeak, if you carefully identify it, you will find that the squeak with different frequencies represents the different mood states of the rats.

"Eat, eat more." Cui Hualin continued to feed his young men fresh fruits and vegetables. He studied in biology and worked for so many years, and has suffered more than one rat life.

The other four brothers had more rats in their hands. They performed many experiments with white rats. They respected these small animals and respected life. If they had to execute the experimental mice, they tried to choose the lowest pain treatment method.

Compared with mice that are responsible for drug experiments, bacteria experiments, virus experiments, radioactive experiments, and pathological experiments, these mice used in IQ gene experiments will hardly feel pain in their short lives.

"Have you eaten?" Han Meng asked the friend in the cage.

Friends replied: "Squeak!"

"Then let's start the experiment?"

"squeak!"

"Thank you for your understanding and for your cooperation."

The five experimenters asked their friends out of the cage and moved them to the biological laboratory next door.

The rats were first anesthetized on the experimental bench, and then the experimenters operated gene editing equipment to perform shank3 gene editing on the rats.

According to the experimental plan he obtained in the in vitro cell-free system, Han Meng conducted biological experiments on the experimental mice he selected.

"Identify the target base..."

"Removal of non-methylated chains..."

"Correct mismatched bases..."

The experiment went smoothly.

The experimental mice were subjected to shank3 gene editing surgery by the experimenters during sleep.

"I'm done."

"I've finished it, too."

"Next, let's look forward to the miracle happening."

I just want to be a quiet student

I just want to be a quiet student
Chapter completed!