The 10 experimental mice from Han Meng's group performed excellently in the T-maze, and Han Meng also conducted further research on his friends.

The five experimenters sorted out the original data of their experimental mice and gave it to Shen Qi.

Shen Qi conducted in-depth analysis of the original data through a combination of mathematics, physics, chemistry and biology.

After the Duyuan group rats modified the shank3 gene, they all died of intracranial hemorrhage in a short period of time.

Why does Du Yuan’s editing plan lead to intracranial hemorrhage in rats?

Shen Qi didn't understand this, and he needed to continue researching it.

Cui Hualin and Tang Yaxing's editing plan made their experimental mice extremely violent or autistic.

Regarding this, Shen Qi analyzed the general reasons.

According to the editing plan of Cui Hualin and Tang Yaxing, after the shank3 gene was modified, a new mutation in the rat gene was triggered in a very short period of time, causing irreversible damage to the rat nervous system.

The above three sets of experiments failed.

From the failure, Shen Qi summarized two words: password.

What is the password? Where is it? How should it be controlled?

The nucleus contains chromosomes, which are composed of DNA and histones.

DNA was considered a protein molecule in the early stage, but later it was confirmed that DNA is actually a DNA molecule.

A gene is a DNA sequence containing genetic information. Its encoding is genetic information. It generates proteins through transcription and translation to express the information it carries.

The growth of an organism is caused by continuous cell division, and during cell mitosis, chromosomes are replicated.

At the same time, if the password is passed, a new password may be generated.

Shen Qi believes that the codebook is hidden in the filament structure of the chromosome, and the code can be written and read. The key question is, how do cells choose histones and what method do they take to write the reading code?

In order to master the precise operating principles of biological codes, Shen Qi first studied genes, followed by DNA and proteins, then chromosomes and cells.

The difficulty in studying genes lies in gene mutations. Shen Qi feels that using pure biological methods cannot solve this problem.

t=te^w/kt

Shen Qi wrote this formula.

This is an unproven formula, which the academic community calls the "mutation possibility formula".

Planck discovered quantum theory in 1900, and DeFries, Collins and Chuchemarck rediscovered Mendel's paper in 1900, and DeFries' paper on mutations published between 1901 and 1903. From this, it can be seen that quantum theory and modern genetics emerged almost simultaneously.

Is there a link between quantum theory and genetics?

Shen Qi believes that connections will only arise when both disciplines develop to a higher level.

To this day, quantum theory and genetics have developed to a higher level.

This led to a new school of science - quantum biology.

Quantum biologists try to explain mutations through quantum theory.

The expected time of mutation in the mutation possibility formula is the t-threshold energy w mutation.

The quantum biology school has not yet proved the universality of t=te^w/kt, so this formula is temporarily called the "mutation possibility formula" rather than the "mutation formula".

"The threshold is the order of magnitude required to explain biological heritable persistence..."

Shen Qi frowned and thought, and he continued to analyze the experimental data of rats in Han Meng's group and Wu Junqiang's group.

Not long after, Wu Junqiang said frowningly: "Director Chen, there is bad news. The IQ of my experimental mice has dropped. They can only walk out of the full right-facing maze, but in other aspects, they become as stupid as fools."

Shen Qi didn't think so: "No, this is not bad news, this is good news."

Wu Junqiang was a little confused: "Is this good news for experimental mice to reduce their IQ through gene editing?"

Shen Qi expressed his point of view: "The so-called regulation should be adjusted by genetic means or low. This is a complete operation process. Of course, we prefer to increase the IQ, but it is also undeniable that the academic value of lowering the IQ. In some specific situations, reducing the IQ of the experimental target may have a greater practical purpose."

Wu Junqiang's body shook: "Suppress the wisdom and strike?"

Shen Qi smiled slightly: "Xiaoqiang, how deep do you know about t=te^w/kt?"

"This is the formula for mutation possibility proposed by the Quantum Biology School. I have some understanding of it. My foreign mentor is obsessed with quantum biology. This is how I understand this formula..."

After some exchanges, Wu Junqiang suddenly realized and began to draft the paper.

The title of the paper is "Study on Rats with Various Shank3 Gene Editing Methods".

The abstract meaning is: "Study shows that the IQ of rodents is affected to a certain extent by the shank3 gene. This research group chose Wistar rats as the experimental subject, and used shank3 gene editing to perform shank3 gene editing through mismatch editing, nucleotide resection editing, base resection editing, homologous recombination editing, and non-homologous end junction editing, and achieved preliminary research progress."

The other four experimenters also joined the ranks of paper writing.

Han Meng wrote in the paper he was responsible for that the IQ of experimental rats was significantly improved through mismatch editing. He also listed the main experimental data and conducted a cause analysis.

Wu Junqiang wrote in the paper he was responsible for that the IQ of experimental rats was reduced by base excision editing. He also listed the main experimental data and conducted a cause analysis.

Cui Hualin, Tang Yaxing and Du Yuan also gave their experimental data and experimental results.

Cui Hualin, Tang Yaxing and Du Yuan could not understand the real reasons for intracranial hemorrhage, rage and autism of the experimental mice. The analysis of the reasons for the failure lessons was written by Shen Qi.

With everyone's diligent efforts, "Study on Rats with Multiple Shank3 Gene Editing Methods" was quickly completed.

It seems a bit hasty, but Shen Qi thinks it can be published first.

There are many types of animal experiments, such as oral or injected drugs in animals, whose experimental cycles are the drug efficacy cycle and the metabolic cycle of animals.

The experiments of gene editing on animals should be the normal life cycle of animals, and even the life cycle of one or several generations of experimental animals should be taken into account.

Under normal circumstances, wistar rats can live for several years.

Then the paper produced by gene editing experiments on wistar rats should not be completed in a few years when the wistar rat dies.

The author of the paper even needs to observe various indicators of the offspring of the Wistar rat before he can finish writing the paper and give hereditary conclusions.

If you do this, it will take at least five or six years.

Shen Qi couldn't wait for that long, and his five doctoral students were not waiting.

So they wrote in the abstract and summary of the paper: "This experiment has achieved preliminary research results. In the next stage, we will continue to observe various indicators of experimental rats and study the operating principle of the shank3 gene more in-depth."

This must be written because the next stage of research conclusions may overturn the preliminary conclusions of this paper.

In the preliminary conclusion, Shen Qi's team gave their core views:

1. The shank3 gene does affect the IQ of rats to a certain extent;

2. Edit the shank3 gene through technical means to adjust the IQ of rats;

3. There are risks in regulating IQ, and there is a high probability that new mutations will be triggered, but the mechanism for triggering new mutations has not been fully understood. Other research teams should not imitate until this team gives the final conclusion.
Chapter completed!