Chapter 190 Please don't take my moon with you(1/2)
No. 2333, Jiangzhou Science and Technology Park, Institute of Cryptococcosis.
It has been more than half a year since its establishment last year.
But so far, the institute has found nothing.
The reason has been mentioned before: cloning cells is too difficult because the content of antigenic B cells in the serum is too low.
After isolation into B cells, the sensitivity and specificity requirements for its sequence amplification and determination are also very high.
Even if the sequence is amplified and the antibody is expressed, the "neutralization effect" of the antibody needs further identification.
In short, it is extremely difficult to replicate antigenic B cells.
Of course, the above are all for ordinary people.
Not including Zhou Wen.
He has now upgraded several disciplines to lv4, and he can almost start trying to do this task.
This task brought Zhou Wen not only experience, but also some additional things, such as influence in the academic world, which other tasks cannot bring to him.
On the afternoon of May 5, Zhou Wen officially started cell cloning research.
The first step is to isolate B cells.
This is extremely difficult for others, but for Zhou Wen, who has the eyes of true vision, it is a piece of cake.
First of all, his eyes could see the antigenic B cells in the serum directly;
Secondly, the upgrade of biological immunology to lv4 brings him not only a simple amount of knowledge, but also a deeper understanding of antigenic B cells. He has integrated a unique separation method.
These techniques are independent of existing biological knowledge.
One week later, Zhou Wen successfully isolated antigenic B cells of cryptococcal disease.
Li Feng and Fan Bo, who had always complained about Zhou Wen, almost glared out their eyes.
The other 13 doctoral researchers in the laboratory were also shocked.
Everyone's admiration for Zhou Wen is like the surging river, continuous, and like the flood of the Yellow River, it is out of control.
But no one cheered!
This is just the first step in the Long March.
Next is the highlight - "sequence amplification"!
This is also a huge difficulty for ordinary people, because antigenic B cells are too rare, and the failure rate during sequence amplification is as high as 99%!
And if you fail, you will probably never have a chance.
But this is not a big problem for Zhou Wen.
Don’t forget, what did Zhou Wen start with? DNA extraction and amplification!
The whole world extracts DNA and PCR. He thinks he is second, but no one dares to claim himself first.
Of course, Zhou Wen did not dare to take it too lightly, so he cheered up and went all out.
Because there are too few specimens.
If it fails, not only will the isolated B cells be invalidated, it will take another week to re-isolate, and it may not be successful in one go!
It is similar to the DNA amplification procedure of mouse, but he redesigned the primers himself.
Several simulation experiments were also conducted.
Six days later, Zhou Wen successfully amplified a sufficient number of antigenic B cells, and after product analysis, it was confirmed that the amplification was successful!
There are no shortage of research samples now.
The whole laboratory was in full bloom!
Everyone looked at Zhou Wen like a god.
If the isolation of antigenic B cells at one time is still in the human category, then if there is no operational experience and no chance of failure to start over, it is completely non-human technology to amplify antigenic B cells at one time.
At this moment, Zhou Wen became their spiritual belief!
Even if he says he is an alien now, they still believe it!
Next is the antibody expression assay.
Antibody expression begins with the transcription from DNA to MRNA and reaches orgasm when MRNA is translated into a protein...
A 30,000-word introduction is saved here.
As expected, the sensitivity of antibody expression is naturally passed at one time.
Li Feng and a group of doctors were not surprised at all, and even thought it was taken for granted.
Next is the most important step and the last step to test the antibody "neutralization effect".
Because the "antibodies" themselves only have the function of identifying target antigens and do not kill or reject target antigens.
The "neutralization effect" is that the antibody binds to the antigen to produce precipitation or cell group, and then is engulfed by the phagocytocytes, thereby killing the antigen completely!
If the neutralization effect test is successful, it means that the vaccine for cryptococcal disease has been successfully manufactured.
...
Xiangya Hospital, Office of the Director of the Department of Infection.
Cheng Yanqing was writing an experimental report when the phone rang on the desk.
She picked it up and looked at it. She was a member of the Virus Exchange Home and was the director of the Infectious Disease Department of Xiehe Hospital.
"What's the matter with Director Pan?"
"Zhou Wen said that the antigenic B cells they amplified have undergone antibody expression assays and will be tested for neutralization effect soon."
Cheng Yanqing was stunned for a moment, then said in shock: "What did you say?"
"I said that Zhou Wen and the others have isolated and amplified antigenic B cells of cryptococcal disease, and the neutralization effect test will be carried out next."
"Impossible!" Cheng Yanqing said firmly, "I called the research institute at the beginning of the month, and it was still doing basic research there, it was impossible."
Pan Renfeng said: "This is not what I said, this is what Zhou Wen said himself, come to the group to take a look."
When Cheng Yanqing heard this, he immediately opened the WeChat group and saw Zhou Wen’s speech.
It was the old house of Xia family who asked Zhou Wen what he was doing recently, so Zhou Wen replied casually that he was cloning antigenic B cells of cryptococcal disease.
Then the old house of the Xia family asked him how he was doing research?
Zhou Wen said that the neutralization effect test will be started soon.
Zhou Wen's words exploded all the big guys in the group who dived and peeped the screen.
Everyone couldn't believe it.
But after Zhou Wen posted a few test data sheets, the group was in an uproar.
Cheng Yanqing clicked on the test data and looked at it, shocked to the point of being added!
She doubted whether it was April Fool's Day?
How could such a thing exist?
She called Li Feng at the beginning of the morning, but he sighed and said that there was no progress, and his words revealed his complaints to Zhou Wen.
But now...
Cheng Yanqing immediately called Zhou Wen, who was on the phone.
Cheng Yanqing did not hang up the phone, but waited.
About a few dozen seconds later, the phone was answered.
"Zhou Wen, what's going on?"
"That... I can't explain clearly for a while. Let's do this. Director Cheng, if you have time, come to Jiangzhou tomorrow and have a face-to-face conversation."
Cheng Yanqing had too many things to ask in his mind, but at this moment he didn't know where to ask, so he had to hang up the phone for the time being.
How could Yan Qing wait until tomorrow if he doesn’t do it?
Without saying a word, he immediately set off for Jiangzhou.
On the way, I called the laboratory leader Li Feng over and over again.
Finally it was opened.
Under Li Feng's story, Cheng Yanqing's chin was almost shocked and he couldn't come back to his senses for a long time.
“Doududu————”
Finally, Cheng Yanqing was awakened by the sound of the horn behind him, and only then did he realize that he had stopped at the traffic light intersection.
Cheng Yanqing took a deep breath after starting, and asked, "Why don't you tell me about such a big thing?"
Li Feng said: "Because Zhou said that the process is not important, what is important is the result. If the neutralization effect test fails, all the sweat and efforts before will be meaningless, so there is no need for everyone to know."
Cheng Yanqing: “…”
To be continued...