Wu Yan knew that Zhou Wen must have a special extraction method.

However, seeing that he was unwilling to say it, she did not force it. After all, this is someone else's unique ability, and it is harmful or useless to leak it out.

Then the two talked about the treatment issue again. Wu Yan told Zhou Wen again what she said to Shi Lei before, and finally asked with a smile: "Do you have any questions?"

Zhou Wen thought of the task and said, "Can you ask me if I am a contract worker or a formal worker?"

"Well..." Wu Yan hesitated for a moment. She didn't expect Zhou Wen to ask this question.

She believed that Zhou Wen should be very clear about the current situation of biology. The contract of Jiangzhou First People's Hospital is so generous. Not to mention that he is a small undergraduate, even if he is a master's and doctoral student, many people will be reluctant to do so.

He actually wanted to get to the top and solve the staffing problem directly, which was a bit too much. She is still a contract worker.

Wu Yan thought for a while and said, "Generally speaking, if it is hired by the hospital, it must be a formal worker, but you are the employment contract of the PCR group of the testing center, so I'm afraid you can only be a contract worker."

"Contract worker..." Zhou Wen hesitated.

In fact, the contract worker is still a formal worker, so he really doesn't care. As long as he can go to the testing center to practice, he will be willing to do it even if he is a temporary worker.

This is not a task that requires becoming a formal worker within one week, so he has to stretch some things.

1000 experience points and 1000 points, and there is also a chance to open a black iron box.

Wu Yan originally thought that the matter had been done, but she didn't expect that there would be troubles here again, so she said with great thoughts: "Although you are a contract worker, your treatment is calculated based on a formal worker, and there is no difference.

In addition, everything is done step by step. You can go to work first, and it is not too late to solve the problem after a while."

Zhou Wen asked with a smile: "You are my senior sister and you have been in the hospital for one year. To be honest, if I really sign a contract worker, when will the establishment be resolved?"

"Well..." Wu Yan was a little embarrassed and picked up the coffee cup to cover it up.

After putting down the cup, Wu Yan said, "Then it, I'll help you go back and ask. But to be honest, don't have too much expectations."

Zhou Wen nodded and smiled and said, "Okay, then I'll bother senior."

"You're welcome."

Zhou Wen was still in a state of reluctance and didn't stay too long. He was so kind that he stood up and left.

...

When they returned to the laboratory, Yang Yudong and Gu Hongyun couldn't wait to ask him if the interview was successful?

Zhou Wen said with a smile: "I made a condition, and she said she would go back and discuss with the leader, and then give me a letter after the discussion."

Yang Yudong said speechlessly: "The conditions are so superior, do you still have to make the conditions?"

Zhou Wen said: "I'm not looking at the rare opportunity, I just want to try their lowest price."

Yang Yudong: “…”

He really wanted to use the extraction gun to pull Zhou Wen's brain out a little, and see what he was thinking all day long?

Zhou Wen laughed and went to do the PCR experiment.

The full name of PCR is polymerase  rea.

The Chinese name is "polymerase chain reaction", also known as "polymerase chain reaction", which is an in vitro nucleic acid amplification technology developed in the mid-1980s.

The gene or DNA fragment to be studied can be amplified to a hundred thousand or even million times within a few hours.

Its principle is basically similar to DNA replication images in human cells, but the reaction system is relatively simple.

Of course, this does not mean that PCR is simple.

On the contrary, PCR is very difficult.

First, through high temperature "denaturation", a double-stranded DNA is melted into two single-stranded DNA.

Then, the "annealing" is made to allow the "primer" and the DNA as the template to complement each other at low temperature to form a local double strand.

Finally, medium-fire "extend" is used to catalyze the DNA chain with primers as the starting point to produce an extension reaction, which becomes a DNA with two double-stranded structures.

Repeat this process again and again.

1 changes 2, 2 changes 4, 4 changes 8, 8 changes 16, 16 changes 32...

Through PCR technology, sufficient DNA can be expanded from a hair, a drop of blood, or even a cell for analysis, research, detection and identification.

For example, paternity test;

For example, vaccine research;

For example, virus disease prevention and control;

Or genetic disease research, etc., all require a lot of PCR.

Of course, there is actually no need to do PCR when Zhou Wen did a rice aphid biochemical reagent experiment today.

Aphids are very low-level insects. They are usually used directly to act on them and then look at their genetic characteristics.

The reason why I still need to do PCR is more of the purpose of learning PCR.

The PCR reaction system includes water, buffer, dntp (deoxyribonucleoside triphosphate), primer p1/p2/dna template and taq enzyme.

After everything was ready, Zhou Wen adjusted the range of the extraction gun, and then extracted 30μl of water into three sets of PCR tubes.

When absorbing liquid, strictly follow the operating procedures, absorb the liquid vertically, and slowly absorb and release it slowly.

After changing the tip of the gun, extract 5 ml of buffer into the PCR tube.

Then add dntp/p1/p2/dna templates to the tubes respectively...

Thanks to this period of study, Zhou Wen was in an orderly manner, except for a little nervous at the beginning.

After the required raw materials are added, centrifugation is next.

After setting the speed as required, waited for three minutes and centrifuged.

Zhou Wen took out the PCR tube containing the mixture from the centrifuge, immediately placed it on the PCR instrument, and amplified it.

Set 94c predetropy for 4 minutes.

Then set 20 cycle amplification stages.

High temperature, low temperature, medium temperature, high temperature...

1 changes 2, 2 changes 4, 4 changes 8, 8 changes 16, 16 changes 32...

Zhou Wen was waiting anxiously, feeling like a husband outside the delivery room, silently praying for "mother and son's safety" in his heart, and was also thinking, I wonder if the man or woman will be born later? Are you healthy or not?

At this moment, Zhou Wen suddenly thought that the system points could speed up the experiment. Instead of waiting here for a few hours, should he take a quick look?

Anyway, no one saw it here.

Thinking of this, Zhou Wen immediately summoned the personal attribute page. After clicking on points, an option popped up in front of him.

[Will you consume points to speed up the experiment progress? Yes/No]

【yes】.

[This task requires a total of 20 points to consume, please confirm again]

Zhou Wen saw that he could speed up the experiment with just 20 points, so what else to hesitate about, so he immediately chose to confirm.

Before Zhou Wen could react, the next second, the PCR instrument had completed the amplification.

Zhou Wen was surprised, the power of the system was simply mysterious.

He sighed in his heart, and soon he became nervous again.

I'm the first time I've done PCR, I wonder if it will be successful?

Zhou Wen opened the PCR instrument and took out the PCR tube from it.

Originally, the following was a product analysis, but Zhou Wen had a true vision and narrowed his eyes slightly. Soon he saw the DNA of the high-density double helix structure.

“perfect~”

If it weren't for wearing a mask, Zhou Wen would have laughed out loud.

There are ones that count on one. Who hasn’t learned to do PCR after countless failures? Who can succeed in the first amplification like him?

And the amplification effect is unexpectedly good, and it is simply perfect.

...

Jiangzhou City, First People's Hospital, Testing Center, PCR Group.

Wu Yan returned to the hospital after lunch, and then went to the team leader's office to report the situation as soon as possible.

"Where is the person?" Zhang Shuguang, who was writing the experimental report, asked without raising his head.

Wu Yan said embarrassedly: "That... hasn't come yet."

When she went there, she patted her chest and promised that she could get it right, but she turned out to be a blowjob.

Zhang Shuguang looked up and said unexpectedly: "Why, don't you want to come?"

Wu Yan shook her head and said, "No, it's just that he said..."

Wu Yan told Zhang Shuguang about Zhou Wen’s request.

Zhang Shuguang held the pen and tapped on the desk with his left hand, and said with a smile: "You said you, such a smart person, why did you lose your chain at a critical moment?"

Wu Yan said strangely: "Why did I lose my link? He wants formal work treatment. How dare I interfere with this? I agree to you?"

Zhang Shuguang smiled and said, "What's wrong with overdoing the business? He is a biology undergraduate. He dares to ask you for a career position. Don't you think about why?"

"Why?" Wu Yan was puzzled, and soon reacted, and said in surprise: "Oh~~~ you mean..."

Zhang Shuguang laughed and said, "You have come to find someone. As long as it is not too outrageous, you can agree to him first. If you have any questions, you will talk about it. Oral promises are not guaranteed, right?"

"Yeah yeah yeah ~ Boss, I know I'm wrong." Wu Yan nodded repeatedly and admitted her mistake, "Then I'll call him now?"
Chapter completed!