Chapter 142 Cultivation of Dinosaur Embryos
"Start simulate chromosomal results...eliminate 520,000 results."
"Start histone binding simulation...each 490,000 results."
"Start to simulate building cell nuclei..." After layer by layer of screening and elimination, Du Xingyu obtained more than 100 gene maps that can build dinosaur cell nuclei.
These maps still have flaws, and they need to simulate, eliminate and correct them one by one.
After another week of work, Du Xingyu finally got a complete Tyrannosaurus Rex gene map.
Relying on this genetic map, he can synthesize genomic DNA, bind to histones, and put it into the nucleus to form a nucleus containing the genetic gene of Tyrannosaurus rex.
This only completed 10% of the cloned Tyrannosaurus Rex. Next, he needed to transplant the cell nucleus into the cells to build "dinosaur cells" and induce it into pluripotent stem cells.
Some of the biotechnology involved in this has exceeded the current technical scope of human beings. Du Xingyu needs to rebuild research projects and conquer them one by one.
...
Time flies and a semester has passed. In mid-January, the school’s winter vacation period.
"Du Xingyu, I'll quit first, see you next year." Bao Xian had already packed up his greetings and was about to go home for the New Year.
"By bye." Du Xingyu said goodbye to him.
In the laboratory, the team members also went home to celebrate the festival.
"Du Xingyu, aren't you going home?" Du Xingyu's mentor Su Yutang said curiously.
"I want to continue to study this topic. There are few people in the laboratory during holidays, so I can use it more." Du Xingyu explained.
As a graduate student, he doesn't really have much time to use laboratory equipment, and many of them are shared by several people. Du Xingyu wants to take advantage of the Chinese New Year to cultivate Tyrannosaurus Rex stem cells, so that Tyrannosaurus Rex embryos can be further cultivated.
Besides, Du Xingyu has no family in this world.
"Academic research is important, and life is equally important." Su Yutang held his granddaughter Su Ziyun with one hand and put the key in his hand with the other, saying: "Your paper on gene sequencing is very well written. You can try to contribute to "Cell"!"
"Well, I will do it if I have time." Du Xingyu said calmly.
Su Yutang looked at him in surprise. "Cell" is one of the most important journals in life sciences, and is ranked alongside "Nature" and "Science".
No matter who I tell you, his paper is qualified to contribute to "Cell", I am afraid that I will be so excited that I can't sleep?
Is this student a bit too calm? It's like telling an ordinary person that he won 100 million in lottery, but this person didn't react at all!
"Do it well!" Su Yutang said.
"Goodbye Brother Xingyu." Su Ziyun said goodbye politely, and she waved and said, "You can definitely study the dinosaur burger!"
Du Xingyu: “…”
Du Xingyu finally had plenty of time to use high-end laboratory equipment and personally built Tyrannosaurus Rex cell nuclei, which was much more difficult than on a technology simulator.
"Hello everyone, this is the Creator VLOG. Today is the 9th video." Du Xingyu was still insisting on updating his video log, although the number of fans has only reached one hundred.
"In the previous stage, I had built the nucleus of Tyrannosaurus Rex, and today I'm going to transplant it into the recipient cells and cultivate real dinosaur cells."
"The operation process is not shown... After several failures, I finally got a mature dinosaur cell."
"This cell needs to continue to be cultivated, divided, and then induce it to become a stem cell."
Du Xingyu posted the video a day after he posted it, and there were only a few comments.
1:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:00:0
FIHJJ: "I haven't seen it for a long time. Is this person still cloning a dinosaur?"
Tomato sauce: "It's almost half a year, why haven't it cloned yet?"
Eye: "Hey, I actually met a hardcore author. I am also a student in the biology department. I feel that the author is very powerful. I'll go and see your previous video!"
...
On New Year's Eve, thousands of lights are on New Year's Eve.
The whole country is shrouded in the joy of family harmony, and Du Xingyu is still studying in the laboratory.
He has cultivated dozens of generations of Tyrannosaurus Rex pluripotent stem cells, constantly inducing, screening and firming, hoping to get a stem cell that can be used to cultivate embryos.
Thanks to the technology simulator, he saved a lot of trial and error costs. If Du Xingyu really tried it slowly alone, I am afraid it will be difficult to complete it in my life!
The technology simulator can eliminate countless error possibilities in advance, and generate simulation results in an instant, which originally took several years or even hundreds of years of experiments!
Under a biological microscope, Du Xingyu finally discovered a dinosaur stem cell that meets the requirements in an in vitro cell culture dish.
"That's great!" Du Xingyu was overjoyed, "You can finally cultivate dinosaur embryos!"
Inducing stem cells to develop into embryos in vitro is also a super difficult thing. But Du Xingyu is not worried at all. He has even cultivated himself into a Tyrannosaurus Rex individual in the technology simulator.
It is just because of the limitations of the actual experimental process, so it has not been completed yet.
"If everything goes well, I will probably be able to complete it in three months." Du Xingyu thought to himself.
He picked up his phone, and the information bar showed New Year greetings from his friends. Du Xingyu edited the message and sent it: "Happy New Year!"
...
The winter vacation has passed and the students have returned to school.
Bao Xian rushed to the laboratory immediately and shouted: "Du Xingyu, Du Xingyu! Where are the dinosaur embryos you have cultivated?"
"Don't tease me. I've had a week off. I'll come here quickly after hearing what you said!"
During a chat not long ago, he learned that Du Xingyu was already nurturing the in vitro embryo of Tyrannosaurus Rex.
"In the embryo culture room." Du Xingyu smiled.
"Let me see!" Bao Xian didn't care about the others and went over to watch immediately.
In the embryo cultivation room, dozens of small embryos are taking shape. Du Xingyu used biological culture solution to simulate the situation of the parent body and cultivated it.
Because dinosaurs have long been extinct, it is difficult to find a suitable surrogate host now. Du Xingyu originally wanted to use birds as surrogate hosts of dinosaur embryos, but he found that the eggs of Tyrannosaurus Rex were too big, and only ostrichs in the world could barely give birth to it.
However, what vertebrates express during development will be affected by the parent's genes and proteins. Du Xingyu used a technology simulator to simulate countless times, and most of them failed.
Only a few times have the simulated surrogate ostrich gave birth to half-bird and half-dinosaur monsters, and these little monsters have died in a few minutes after they break their shells.
So after comprehensive consideration, Du Xingyu chose to completely simulate the dinosaur mother's situation and provide a developmental environment for Tyrannosaurus Embryology.
"Is this true?" Bao Xian found it difficult to believe that these embryos are Tyrannosaurus Rex's embryos. These embryos have just begun to develop, and it will take dozens of days to develop into a Tyrannosaurus Rex egg.
"Of course it's true," Du Xingyu said, "but I expect that in the end, only three or five of the dozens of samples can develop into eggs. There should be only one or two that can hatch successfully."
Du Xingyu is conservative. According to the simulation results on the technology simulator, the survival rate of these embryos should be higher.
"Damn, don't tease me. If this is a Tyrannosaurus Rex embryo, then you will be bigger!" Bao Xian said, "You have done what scientists around the world have not done!"
"I'm not sure yet." Du Xingyu said.
"No, I still don't believe it!" Bao Xian shook his head repeatedly, "Even if you find the Tyrannosaurus Rex gene map, it is impossible for it to encode its nucleus and stem cell cultivation in such a short time... Have you never made any mistakes at once?"
"How much workload does this have and how many global problems can you complete it alone?"
"Is this another animal embryo? Is this a secret change of topic?"
"It's up to you to believe it or not." Du Xingyu couldn't explain, so he couldn't tell him that he would carry a black technology plug-in with him, right?
...
"Are these really Tyrannosaurus Rex embryos?" Not only Bao Xian, but other members of the laboratory were also puzzled.
Pang Yan said in confusion: "It makes no sense, this..."
"Junior brother, is this another animal embryo? It seems to be some kind of bird." said a senior sister.
"Du Xingyu, you teased us as soon as we came." Yang Mu said, "I don't believe it can really cultivate a Tyrannosaurus Rex!"
Du Xingyu said helplessly: "The embryo has just begun to develop... It will be seen in a while."
"Your Tyrannosaurus Rex gene map cannot be proven to be true at all! There are no living Tyrannosaurus Rex in the world!" someone said.
"Impossible, impossible!" said a doctoral senior brother named Zhu Lianbo, "If this is a dinosaur embryo, I can believe it to you!"
"Don't believe me." Du Xingyu said, "This way, if I really cultivate dinosaur embryos, you can just treat me to a meal. If I fail, I will treat everyone to a meal."
"Okay!" Yang Mu said, "That's it!"
"Okay, then you have to cultivate it well!" said Pang Yan, Zhu Lianbo and others.
The incident of Du Xingyu cultivating dinosaur embryos not only surprised and puzzled the people in the entire laboratory, but even Su Yutang took a look at it in person.
He carefully looked at some of Du Xingyu's experimental records and said very seriously: "Du Xingyu, no matter whether this experiment can be successful or not, you have already said that it has been successful!"
"I encode the genome by myself and induce stem cells to cultivate embryos. This technology alone is enough to alarm the entire biological world!"
"From now on, your project is the most preferred project in our laboratory, and all experimental projects make way for it!"
"I hope you can produce more research results!"
"Thank you, teacher!" Du Xingyu thanked.
Chapter completed!